Evaluation of contribution of nasunin to ORAC value and hyaluronidase inhibitory activity of nasunin-containing powder obtained from spent eggplant pre-pickling solution
2017
Itoh, K. (Industrial Technology Center of Tochigi Prefecture, Utsunomiya, Tochigi (Japan)) | Akutsu, S. | Watanabe, T. | Xie, X. | Komatsu, W. | Yoshizawa, F. | Uda, Y.
Nasunin was recovered from spent eggplant pre-pickling solution with 96 % purity by gel-filtration on an LH-20 column and subsequent preparative HPLC. The purified nasunin preparation was analyzed by LC-MS/MS and found to contain a mixture of cis- and trans-isomers of nasunin. The purified nasunin preparation was then examined for antioxidant activity by measuring the H-ORAC value. Hyaluronidase inhibitory activity of the purified nasunin preparation was also determined. H-ORAC value of the purified nasunin preparation was estimated to be 13 250 micromol Trolox equivalent/g, corresponding to 1.45 times higher than that of the delphinidin standard. However, the contribution of nasunin to the Trolox equivalent/g of the nasunin-containing powder was estimated to be approximately 26 %, which suggests that constituents other than nasunin, such as chlorogenic acid, play a significant role in the antioxidative activity of the nasunin-containing powder. The purified nasunin preparation showed no inhibitory activity of hyaluronidase, but chlorogenic acid, another component of the nasunin-containing powder, had a strong inhibitory activity. Therefore, the inhibitory activity previously observed with a cyclodextrin-inclusion powder of the nasunin-containing powder from spent eggplant pre-pickling solution appears to be attributable to the activity of the chlorogenic acid-containing constituents other than nasunin therein.
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