Natural diversity of seed alpha-tocopherol ratio in wild soybean (Glycine soja) germplasm collection
2016
Dwiyanti, M.S. (International Rice Research Institute, Los Banos, Laguna (The Philippines). Plant Breeding Division, Genotyping Services Laboratory) | Maruyama, S. | Hirono, M. | Sato, M. | Park, E. | Yoon, S.H. | Yamada, T. | Abe, J.
Alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin E activity in humans. Most cultivated soybean seeds contain gamma-tocopherol as the predominant form, and the ratio of alpha-tocopherol content to total tocopherol content (alpha-tocopherol ratio) is <10%. Three soybean accessions from Eastern Europe have alpha-tocopherol ratios of >20%. This higher content is likely due to mutations in the promoter region of the gamma-tocopherol methytransferase-3 (gamma-TMT3) gene. We surveyed a wild soybean germplasm collection and detected 16 accessions with stable seed alpha-tocopherol ratios of >20% under different growth conditions. The alpha-tocopherol ratios were greatly reduced when the plants were grown under cool temperatures during seed maturation, but increased to varying degrees at higher temperatures. Sequence analysis of the gamma-TMT3 promoter of 11 of the accessions identified four haplotypes, one of which corresponded to that of cultivars with higher contents. These wild accessions can thus serve as novel donors for breeding cultivars with high alpha-tocopherol ratios and for better understanding the genetic basis of alpha-tocopherol synthesis in soybean.
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