Percentage dynamics of CD4-1sup(+) and CD8alphasup(+) T cells in different tissues at various developmental stages in ginbuna crucian carp Carassius auratus langsdorfii
2018
Khallaf, M.A. (Sadat City University, Sadat City (Egypt). Faculty of Veterinary Medicine) | Ogawa, S. | Toda, H. | Matsuura, Y. | Miyazawa, R. | Nakanishi, T.
Determination of numbers of T cell subsets in tissues is important to assess the functional maturity of the immune system. The distribution of T cells in lymphoid tissues during ontogenic development has been examined in several fish species using pan-T cell markers. However, information on the age-related changes in the number of T cell subsets in tissues is limited due to the lack of antibodies against CD4 and CD8 in fish. In the present study we examined the percentage dynamics of CD4-1sup(+) and CD8alphasup(+) cells in various tissues accompanied with age of ginbuna crucian carp using flow cytometry. We found rapid increases in both CD4-1sup(+) and CD8alphasup(+) T cells up until 6 months post-hatch (mph). CD4 and CD8 double-positive cells (DP cells) are present only in the thymus and DP cells increased, while double negative cells decreased, in fish up to 3-6 mph. The percentages of CD4-1sup(+) T cells were always higher than those of CD8alphasup(+) T cells in the various tissues examined, except for the thymus and intestine. In contrast, significantly higher percentages of CD8alphasup(+) T cells than CD4-1sup(+) T cells were found in the intestine during the period between 6 mph and 2 years post-hatch.
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