Acid-base balance of the hemolymph in hard-shelled mussel Mytilus coruscus in normoxic conditions
2017
Handa, T. (National Fisheries University, Shimonoseki, Yamaguchi Pref. (Japan). Department of Applied Aquabiology) | Araki, A. | Yamamoto, K.
We examined hemolymph pH, total CO2 content (Tsub(co2)), CO2 partial pressure (Psub(co2)) and bicarbonate concentration ([HCO3sup(-)]) in order to evaluate the acid-base balance of the hard-shelled mussel Mytilus coruscus in normoxic conditions. The hemolymph was collected anaerobically through a cannula by pretreatment of the adductor muscle by catheterization. The mean values of the hemolymph pH and Tsub(co2) were 7.617 and 1.44 mM/l, respectively. The CO2 solubility coefficient (alpha sub(co2)) was 40.6 micro M/l/mmHg. The apparent dissociation constant of carbonic acid (pKapp) was able to be expressed using the estimated equation as follows: pKapp=-6371.321+3923.163.pH-856.100.pHsup(2)+82.978.pHsup(3)-3.014.pHsup(4). Using alpha sub(co2) and pKapp determined in this study, hemolymph Psub(co2) and [HCO3sup(-)] were calculated as 0.57 mmHg and 1.42 mM/l, respectively. The non-bicarbonate buffer value (betasub(NB)) was 0.44 Slykes.
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