Disruption of the gene trx-m1 impedes the growth of Anabaena sp. PCC 7120 under nitrogen starvation
2019
Deschoenmaeker, F. (Tokyo Institute of Technology, Yokohama (Japan). Institute for Innovative Research, Laboratory for Chemistry and Life Science) | Mihara, S. | Niwa, T. | Taguchi, H. | Nomata, J. | Wakabayashi, K.I. | Hisabori, T.
Cyanobacteria possess a sophisticated photosynthesis-based metabolism with admirable plasticity. This plasticity is possible via the deep regulation network, the thiol-redox regulations operated by thioredoxin (hereafter, Trx). In this context, we characterized the Trx-m1-deficient mutant strain of Anabaena sp., PCC 7120 (shortly named A.7120), cultivated under nitrogen limitation. Trx-m1 appears to coordinate the nitrogen response and its absence induces large changes in the proteome. Our data clearly indicate that Trx-m1 is crucial for the diazotrophic growth of A.7120. The lack of Trx-m1 resulted in a large differentiation of heterocysts (>20% of total cells), which were barely functional probably due to a weak expression of nitrogenase. In addition, heterocysts of the mutant strain did not display the usual cellular structure of nitrogen-fixative cells. This unveiled why the mutant strain was not able to grow under nitrogen starvation.
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