Bioassay for detection of transgenic soybean seeds tolerant to glyphosate.
2003
TORRES, A. C. | NASCIMENTO, W. M. | PAIVA, S. A. V. | ARAGÃO, F. A. S. DE | Antonio Carlos Torres, CNPH; Warley Marco Nascimento, CNPH; Sonia Alessandra Vasconcelos Paiva, CNPH; Fernando Antonio Souza de Aragão, CNPH.
Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 mM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots.
Show more [+] Less [-]Título em português: Bioensaio para detecção de sementes de soja transgênicas tolerantes ao glifosato.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Empresa Brasileira de Pesquisa Agropecuária