猞猁肠道紊乱及其病因探讨
2015
赵玲玲
为了研究猞猁肠道菌群结构,筛选出致病菌,南京市红山森林动物园采集了4只猞猁的粪便样本(2只正常,2只生病),事先对粪便进行预处理,用酚-氯仿法提取基因组DNA,以此作为PCR反应模板,扩增16S rRNA基因V3区,并将扩增产物进行DGGE电泳,以筛选粪便样本的差异菌群。通过Quantity one软件对DGGE图谱进行相似度分析,发现非疾病样本之间的相似度为0.92,疾病样本之间的相似度为0.96,非疾病和疾病样本之间的相似度为0.65～0.67。由此可以看出两种猞猁粪便样本的微生物群落之间存在明显的差异。
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
Publisher
Editorial Department of Chinese Journal of Wildlife
Other Subjects
Dgge
Language
Chinese
2025-01-28
2025-10-24
DOAJ
Data Provider
This bibliographic record has been provided by Directory of Open Access Journals
If you notice any incorrect information relating to this record, please contact us at [email protected]