Application of a biomarker battery for the evaluation of the sublethal effects of pollutants in the earthworm Eisenia andrei.
2007
Gastaldi, Laura | Ranzato, Elia | Capri, Flavia | Hankard, Peter | Pérès, Guénola | Canesi, Laura | Viarengo, Aldo | Pons, Giovanni | Dipartimento di Sienze dell'Ambiente e della Vita (DiSAV) ; Università degli Studi del Piemonte Orientale - Amedeo Avogadro (UPO) | Dipartimento di Scienze dell'Ambiente e della Vita (DiSAV) ; Università degli Studi del Piemonte Orientale - Amedeo Avogadro (UPO) | Centre for Ecology and Hydrology (CEH) ; Centre for Ecology and Hydrology | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des sciences de l'environnement de Rennes (OSERen) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Università degli studi di Genova = University of Genoa (UniGe)
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Show more [+] Less [-]English. We applied a battery of biomarkers in the earthworm Eisenia andrei to assess the pollutant-induced stress syndrome. Earthworms were exposed to sublethal concentrations of copper (1–10 μM) and benzo[a]pyrene (0.01–10 μM) as models of inorganic and organic pollutants for 1, 3 and 7 days. Four potential biomarkers of stress were measured: lysosomal membrane stability of coelomocytes, lysosomal accumulation of lipofuscin in chloragogenous tissue and of neutral lipids in coelomatic cells, plasma membrane Ca2+-ATPase activity in the intestinal epithelium; metallothionein content was also evaluated as a biomarker of exposure. Significant changes were observed in the parameters measured in earthworms exposed to both contaminants. Certain biomarkers, such as lysosomal membrane stability and Ca2+-ATPase activity, that showed a decreasing trend with increasing pollutant concentration and time of exposure, proved to be particularly suitable to describe the evolution of a stress syndrome from its early phase to the development of pathological conditions. On the other hand, contaminant-induced changes in lysosomal lipofuscin and neutral lipid accumulation showed a bell-shaped trend, indicating that these biomarkers are able to follow the development of pollutant-induced stress syndrome as far as an equilibrium in the cell functions is maintained; therefore, they are particularly useful to describe mild stress conditions.
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