Candida albicans strains adapted to the mouse gut are resistant to bile salts via a Flo8-dependent mechanism
2024
Hidalgo-Vico, Susana | Prieto, Daniel | Alonso-Monge, Rebeca | Román, Elvira | Maufrais, Corinne | D'Enfert, Christophe | Pla, Jesús | Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM) | Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity (BPF) ; Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB ; Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité) | This work was supported in the laboratory of JP by Ministerio de Ciencia e Innovación [grant number PID2021-122648NB-I00]; and Comunidad de Madrid [grant number PR38/21-32] and in the laboratory of CdE by the Agence Nationale de Recherche [ANR-10-LABX-62-IBEID]. We are grateful to S. Azebi for genome sequencing (Biomics Platform, C2RT, Institut Pasteur, Paris, France) supported by France Génomique [ANR-10-INBS-09-09] and IBISA. | ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
International audience
Show more [+] Less [-]English. Candida albicans normally colonizes the human gastrointestinal tract as a commensal. Studying fungal factors involved in colonizing the mammalian gastrointestinal tract requires mouse models with altered microbiota. We have obtained strains of C. albicans through microevolution in the mouse gut for a prolonged period (one year) that display a substantial increase in fitness in this niche. These strains show resistance to bile salts, an increase in their adhesion to the intestinal mucosa, and are unable to filament in response to serum. Genetic analysis revealed some alterations, mainly a triploidy of chr7, a whole chr6 homozygosis, and an SNP in the FLO8 gene (located in the chr6), resulting in a truncated protein version. A wild type FLO8 gene complemented filamentation and bile salt sensitivity but showed an intermediate fitness phenotype in colonization. Alterations in bile salt sensitivity were also evident in bmt mutants, defective in β-mannosylation, and transcriptional targets of Flo8, suggesting a link between the fungal cell wall and mammalian gut colonization via the Flo8 transcriptional regulator.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique