Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant.
2013
Sudre, Damien | Gutierrez-Carbonell, Elain | Lattanzio, Giuseppe | Gaymard, Frédéric | Wohlgemuth, Gert | Fiehn, Oliver | Alvarez-Fernández, Ana | Zamarreño, Angel M | Bacaicoa, Eva | Duy, Daniela | García-Mina, Jose-María | Abadía, Javier | Philippar, Katrin | López-Millán, Ana-Flor | Briat, Jean-François | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Department of Plant Nutrition ; Estación Experimental de Aula Dei (EEAD) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC)-Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | Department of Molecular and Cellular Biology & Genome Center ; University of California [Davis] (UC Davis) ; University of California (UC)-University of California (UC)-Health Sciences Drive, Davis | Centro de Investigadón en Produedón Animal y Vegetal (CIPAV) ; Timac Agro España-Poligono Arazuri-Orcoyen | Plant Biochemistry and Physiology ; Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München (LMU) | European Transnational Cooperation (Germany, France, Spain) within the PLANT-KBBE Initiative; Bundesministerium fur Bildung und Forschung (BMBF) [FKZ:0315458A]; Spanish Ministry of Economy and Competitivity [MINECO EUI2008-03618]; Agence Nationale de la Recherche [ANR-08-KBBE-009-01]; MINECO [AGL2010-16515]
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Show more [+] Less [-]English. Iron homeostasis is an important process for flower development and plant fertility. The role of plastids in these processes has been shown to be essential. To document the relationships between plastid iron homeostasis and flower biology further, a global study (transcriptome, proteome, metabolome, and hormone analysis) was performed of Arabidopsis flowers from wild-type and triple atfer1-3-4 ferritin mutant plants grown under iron-sufficient or excess conditions. Some major modifications in specific functional categories were consistently observed at these three omic levels, although no significant overlaps of specific transcripts and proteins were detected. These modifications concerned redox reactions and oxidative stress, as well as amino acid and protein catabolism, this latter point being exemplified by an almost 10-fold increase in urea concentration of atfer1-3-4 flowers from plants grown under iron excess conditions. The mutant background caused alterations in Fe-haem redox proteins located in membranes and in hormone-responsive proteins. Specific effects of excess Fe in the mutant included further changes in these categories, supporting the idea that the mutant is facing a more intense Fe/redox stress than the wild type. The mutation and/or excess Fe had a strong impact at the membrane level, as denoted by the changes in the transporter and lipid metabolism categories. In spite of the large number of genes and proteins responsive to hormones found to be regulated in this study, changes in the hormonal balance were restricted to cytokinins, especially in the mutant plants grown under Fe excess conditions.
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