Point-of-need dna testing for detection of foodborne pathogenic bacteria
2019
Vidic, Jasmina | Vizzini, Priya | Manzano, Marisa | Kavanaugh, Devon | Ramarao, Nalini | Zivkovic, Milica | Radonic, Vasa | Knezevic, Nikola | Giouroudi, Ioanna | Gadjanski, Ivana | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Université Paris Saclay (COmUE) | Università degli Studi di Udine - University of Udine [Italie] | University of Belgrade | University of Novi Sad
International audience
Show more [+] Less [-]English. Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
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Bibliographic information
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