Metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the European sea bass (Dicentrarchus labrax)
2013
Fernandes, Denise | Dimastrogiovanni, Giorgio | Blázquez, Mercedes | Porte Visa, Cinta
This study investigates the metabolism and mode of action of galaxolide (HHCB) in the European sea bass -Dicentrarchus labrax- following a single intraperitoneal injection of 50 mg HHCB/kg body weight. In addition, a group of fish was injected with 50 mg/kg of ketoconazole (KCZ), a fungicide that is known to interfere with different Cyp isoenzymes. HHCB was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. Both, HHCB and a hydroxylated metabolite were detected in bile. The fungicide ketoconazole was a strong inhibitor of Cyp11β and Cyp3a-catalyzed activities. The work contributes to the better understanding of the impact of synthetic musks on fish and proposes the determination of HHCB and/or its hydroxylated metabolite in bile as a tool to assess environmental exposure in wild fish. © 2012 Elsevier Ltd. All rights reserved.
Show more [+] Less [-]This study was supported by the Spanish Ministry of Science and Innovation under Project CGL2008-01888/BOS and CSIC project PIE 200930I037. Denise Fernandes acknowledges a postdoctoral fellowship (SFRH/BPD/34289/2006) from the Portuguese Fundaçao para a Ciencia e Tecnologia (FCT), and Giorgio Dimastrogiovanni a predoctoral fellowship (BES-2009-025271) from the Spanish Ministry of Science and Education.
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