Enzymatic synthesis and characterization of chlorophyllide derivatives as possible internal standards for pigment chromatographic analysis
2020
Gavalás-Olea, Antonio | Sanz Pereira, Noelia | Riobó, Pilar | Garrido, J. L. | Vaz, Belén
11 pages, 8 figures, 2 tables
Show more [+] Less [-]In this article the chlorophyllase activity of Dunaliella salina has been employed to generate different chlorophyllide a and b esters that could potentially be used as internal standards in pigment analysis. Chlorophyllide a (8’-hydroxyoctyl) ester was selected due its adequate chromatographic and spectral properties and was fully characterized by UV-Vis, ESI-MS and NMR. An easy room temperature procedure for its synthesis is described. Attachment
Show more [+] Less [-]This research was co-funded by the Spanish Ministerio de Economía y Competitividad (MINECO) and FEDER through project CTM2012-32181. This work is appended as part of A. Gavalás-Olea DO*MAR Phd thesis (University of Vigo), who also acknowledge his doctoral FPI grant (BES-2013-065752)
Show more [+] Less [-]Peer reviewed
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