Shotgun proteomic analyses of Pseudomonas species isolated from fish products

12 pages, 2 tables, 4 figures.-- Under a Creative Commons license

Numerous Pseudomonas species can infect aquatic animals, such as farmed rainbow trout, sea trout, sea bass, and sea bream, by causing disease or stress reactions. In aquaculture facilities, a number of Pseudomonas species have been isolated and identified as the main pathogens. The present study describes the characterization of 18 Pseudomonas strains, isolated from fish products using shotgun proteomics. The bacterial proteomes obtained were further analyzed to identify the main functional pathway proteins involved. In addition, this study revealed the presence of 1015 non-redundant peptides related to virulence factors. An additional 25 species-specific peptides were identified as putative Pseudomonas spp. biomarkers. The results constitute the largest dataset, described thus far for the rapid identification and characterization of Pseudomonas species present in edible fish; furthermore, these data can provide the basis for further research into the development of new therapies against these harmful pathogens

Abril A. G. thanks the USC for her “Convocatoria de Recualificación do Sistema Universitario Español-Margarita Salas” postdoc grant under the “Plan de Recuperación Transformación” program funded by the Spanish Ministry of Universities with European Union's NextGeneration EU funds. This work has received financial support from the Xunta de Galicia and the European Union (European Social Fund–ESF), the Spanish Ministry of Economy and Competitivity Project AGL 2.013-48.244-R and the European Regional Development Fund (ERDF) (2007–2013). The study was also supported by the GAIN-Xunta de Galicia Project (IN607D 2017/01) and the Spanish AEI/EU-FEDER PID2019-103845RB-C21 project. The study was also supported by the Plan Complementario en Ciencias Marinas (PCCM) funded by the Ministry of Science and Innovation (Activity 3.6.B. NANOSEAOMICS)

Peer reviewed