Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS Ion Monitoring mass spectrometry
2012
Carrera, Mónica | Cañas, Benito | Gallardo, José Manuel
10 páginas, 5 figuras, 2 tablas
Show more [+] Less [-]Parvalbumins beta (β-PRVBs) are considered the majorfishallergens. A new strategy for the rapid and directdetection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the directdetection of the presence of fish β-PRVBs in any food product in less than 2 hours. This new affordable assay would be very useful for the sanitary and inspect authorities to protect sensitive consumers from allergic reactions and to guarantee their safety
Show more [+] Less [-]This work was supported by the Comisión Interministerial de Ciencia y Tecnología (CICyT) (Project AGL2000-0440-P4-02). Dr Mónica Carrera is supported by the Barrié Fundation
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