Nodulated White Lupin Plants Growing in Contaminated Soils Accumulate Unusually High Mercury Concentrations in Their Nodules, Roots and Especially Cluster Roots

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Two white lupin (Lupinus albus L.) cultivars were tested for their capacity to accumulate mercury when grown in Hg-contaminated soils. Plants inoculated with a Bradyrhizobium canariense Hg-tolerant strain or non-inoculated were grown in two highly Hg-contaminated soils. All plants were nodulated and presented a large number of cluster roots. They accumulated up to 600 μg Hg g−1 DW in nodules, 1400 μg Hg g−1 DW in roots and 2550 μg Hg g−1 DW in cluster roots. Soil, and not cultivar or inoculation, was accountable for statistically significant differences. No Hg translocation to leaves or seeds took place. Inoculated L. albus cv. G1 plants were grown hydroponically under cluster root-promoting conditions in the presence of Hg. They accumulated about 500 μg Hg g−1 DW in nodules and roots and up to 1300 μg Hg g−1 DW in cluster roots. No translocation to the aerial parts occurred. Bioaccumulation factors were also extremely high, especially in soils and particularly in cluster roots. To our knowledge, Hg accumulation in cluster roots has not been reported to date. Our results suggest that inoculated white lupin might represent a powerful phytoremediation tool through rhizosequestration of Hg in contaminated soils. Potential uptake and immobilization mechanisms are discussed.

This research was funded by Agencia Estatal de Investigación, AEI, Spain, grant number AGL2017-88381-R, and by Agencia Estatal Consejo Superior de Investigaciones Científicas, CSIC, grant number 2016SU0005.

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