Infection mechanisms of lichenicolous fungi studied by various microscopic techniques
2002
Ríos, Asunción de los | Ascaso, Carmen | Grube, M.
9 páginas, 10 figuras,
Show more [+] Less [-]Infections of lichenicolous fungi are studied at the cellular level using different microscopic techniques. Confocal laser scanning microscopy of samples stained with Acridine Orange allows the differentiation of hyphae pertaining to a lichenicolous fungus versus hyphae of the host mycobiont. This is similarly possible by hybridizing rRNA in hyphae of a particular symbiont with a specific, labelled DNA fragment. Transmission electron microscopy was used to study details of the interactions among symbionts including the structure of haustoria or ultrastructural features of parasitized cells. The investigations reveal a diversity of infection mechanisms of lichenicolous fungi.
Show more [+] Less [-]The authors gratefully acknowledge Fernado Pinto (Madrid) for darkroom work. Financial support was provided by the Austrian Science Foundation (FWF -P 11998-Gen and DGICYT PB98-0679)
Show more [+] Less [-]Peer reviewed
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