Influence of radiation on Hemarthria compressa's genetic variations
2018
Wu, Bingchao | Zhang, Yu | Yan, Haidong | Yang, Shengting | Zhou, Sifan | Zhang, Xinquan | Liu, Wei | Nie, Gang | Peng, Yan | Ma, Xiao | Yan, Yanhong | Li, Zhou | Huang, Linkai | Sichuan University
Using the material of Hemarthria compressa (L.F.) R.Br. cv. YA’AN, we carried out this research to study the influence of radiation on the genetic variation of plants. Genetic difference was analyzed with expressed sequence tag-simple sequence repeat (EST-SSR) molecular marker through the comparison of 60Co-γ radiation on H. compressa seed stems and original variety. By using 20 primer pairs, 176 polymerase chain reaction (PCR)-amplifications with clear and consistent bands were obtained. The results showed that 155 of 176 bands were polymorphic, which indicating an 88.07% polymorphism rate, and each pair of primers had 8.8 amplified bands on average; the amplitude of polymorphism information content was 0.4709–0.6952 with an average value 0.6081. The genetic similarity coefficient of H. compressa and its mutants ranged from 0.4318 to 0.8239 with an average of 0.6671. As a consequence, existence of genetic differences between the mutants and the basic material was proved.
Show more [+] Less [-]We gratefully acknowledge financial support from the Modern Agro-industry Technology Research System (CARS-34) and the Sichuan Province Breeding Research grant (2016NZ0098-11).
Show more [+] Less [-]Peer reviewed
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Consorcio CSIC-IRTA-UAB Centro de Investigación Agrogenómica (CRAG)