Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira, Dyoni M.; Mota, Thatiane R.; Salatta, Fábio V.; Sinzker, Renata C.; Končitíková, Radka; Kopečný, David; Simister, Rachael; Silva, Mariana; Goeminne, Geert; Morreel, Kris; Rencoret, Jorge; Gutiérrez Suárez, Ana; Tryfona, Theodora; Marchiosi, Rogério; Dupree, Paul; Río Andrade, José Carlos del; Boerjan, W.; McQueen-Mason, Simon J.; Gómez, Leonardo D.; Ferrarese-Filho, Osvaldo; Santos, Wanderley D. dos; Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Czech Science Foundation; European Commission; Ministerio de Economía y Competitividad; Agencia Estatal de Investigación; Ministry of Education, Youth and Sports; Oliveira, Dyoni M. [0000-0002-4871-098X]; Mota, Thatiane R. [0000-0002-7537-3368]; Končitíková, Radka [0000-0002-4398-8982]; Kopečný, David [0000-0002-4309-4284]; Rencoret, Jorge [0000-0003-2728-7331]; Gutiérrez Suárez, Ana [0000-0002-8823-9029]; Tryfona, Theodora [0000-0002-1618-3521]; Marchiosi, Rogério [0000-0002-8386-2524; Dupree, Paul [0000-0001-9270-6286; Río Andrade, José Carlos del [0000-0002-3040-6787]; Boerjan, W. [0000-0003-1495-510X]; McQueen-Mason, Simon J. [0000-0002-6781-4768]; Gómez, Leonardo D. [0000-0001-6382-9447]; Ferrarese-Filho, Osvaldo [0000-0002-3477-4544]; dos Santos, Wanderley D. [0000-0002-6072-2860]; Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

2020

20 páginas.- 8 figuras.- 4 tablas.- 77 referencias.- Additional supporting information may be found online in the Supporting Information section at the end of this article or in https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fpce.13805&file=pce13805-sup-0001-supinfo.docx

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Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored. Here, we conducted an in‐depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants. Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems. The extraction and analysis of arabinoxylans by size‐exclusion chromatography, 2D‐NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt‐stressed roots. Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants. Determination of lignin content and composition by nitrobenzene oxidation and 2D‐NMR confirmed the increased incorporation of syringyl units in lignin of maize roots. Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis. The UHPLC–MS‐based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3‐ and 4‐O‐feruloyl quinate. In conclusion, we present a model for explaining cell wall remodeling in response to salinity.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico, Grant/Award Number: GM/GD ‐ 141076/2016‐0; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Grant/Award Numbers: PDSE ‐ 88881.188627/2018‐01, PDSE ‐ 88881.188639/2018‐01; Czech Science Foundation, Grant/Award Number: 18‐07563S; European Regional Development Fund; Ministerio de Economía y Competitividad, Agencia Estatal de Investigación, Grant/Award Number: AGL2017‐83036‐R; Ministry of Education, Youth and Sports of the Czech Republic, Grant/Award Number: CZ.02.1.01/0.0/0.0/16_019/0000827

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Peer reviewed

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