Haplosporidium pinnae Parasite Detection in Seawater Samples
2023
Moro-Martínez, Irene | Vázquez-Luis, Maite | García-March, José Rafael | Prado, Patricia | Mičić, Milena | Catanese, Gaetano | Ministerio para la Transición Ecológica y el Reto Demográfico (España) | Fundación Biodiversidad
In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.
Show more [+] Less [-]This research was partially funded by EsMarEs (Ministerio para la Transición Ecológica, Spanish Government), and the European Union’s LIFE programme through the projects LIFE INTEMARES (LIFE15 IPE ES 012, coordinated by the Biodiversity Foundation of the Ministry for the Ecological Transition and the Demographic Challenge) and LIFE PINNARCA (NAT/ES/001265).
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