Molecular characterisation of Sardina pilchards larvae diet in Málaga Bay (SW Mediterranean Sea)
2016
Hernández-de-Rojas, Alma | Castro-Pérez, M.C. (María del Carmen) | Cortés-Delgado, M.D. (María Dolores) | Mercado-Carmona, J.M. (Jesús Mariano) | Laiz-Carrión, R. (Raúl) | García-García, A. (Alberto) | Gómez-Jakobsen, F.J. (Francisco José) | Quintanilla-Hervás, J.M. (José María) | Salles, S. (Soluna) | Uriarte-Ramalho, A. (Amaya) | Yebra, L. (Lidia)
The study of predation in the early life stages of fish by direct observation of their stomach content is very difficult, mostly because prey lose all identifiable characteristics. We developed a multiplex-PCR based method, which detected within the gut of the larvae the presence/absence of the five main species of copepods that live in Malaga Bay: Clausocalanus parapergens, Oncaea waldemari, Paracalanus indicus, Temora stylifera and Acartia clausi. A set of five species-specific primers were designed and combined in a single multiplex PCR system, which allowed a time and cost effective screening of the samples. The method detected as little as 0.09 ng/μL of copepod DNA without cross-reactions with Sardina pilchardus DNA.
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