Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs
2021
Gandasegui, Javier | Grau-Pujol, B. | Cambra Pelletjá, María | Escola, V. | Demontis, M. A. | Cossa, A. | Jamine, J.C. | Balaña-Fouce, Rafael | Van Lieshout, L. | Muñoz, J. | Martínez Valladares, María | European Commission | Fundación Ramón Areces | Ministerio de Economía y Competitividad (España) | Junta de Castilla y León | Martínez Valladares, María [0000-0002-3723-1895]
12 páginas, 3 tablas, 3 figuras.
Show more [+] Less [-]There is an urgent need for an extensive evaluation of benzimidazole efcacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the stool sample processing methodology and resistance allele frequency assessment in Trichuris trichiura and Necator americanus anthelmintic-related SNPs by pyrosequenc‑ ing, and standardized it for large-scale benzimidazole efcacy screening use.
Show more [+] Less [-]This study was funded by the ‘Stopping Transmission Of intestinal Parasites (STOP)’ project (EDCTP2 programme supported by the European Union; RIA2017NCT-1845-STOP). JG was personally supported at the beginning of the work by the Ramón Areces Foundation and is now funded by the Spanish ‘Juan de la Cierva’ Programme, Ministry of Economy and Competitiveness (FJC-2018-38305). MCP is supported by the Junta de Castilla y León and Fondo Social Europeo, and MMV by the Spanish ‘Ramón y Cajal’ Programme, Ministry of Economy and Competitiveness (RYC-2015-18368).
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