Small protein mediates inhibition of ammonium transport in Methanosarcina mazei-an ancient mechanism?
2023
Habenicht, Tim | Weidenbach, Katrin | Velazquez-Campoy, Adrian | Buey, Rubén M. | Balsera, Mónica | Schmitz, Ruth A. | Ministerio de Ciencia e Innovación (España) | German Research Foundation | Buey, Rubén M. [0000-0003-1263-0221] | Balsera, Mónica [0000-0002-5586-6050] | Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
17 p., 8 figuras
Show more [+] Less [-]Small proteins containing fewer than 70 amino acids, which were previously disregarded due to computational prediction and biochemical detection challenges, have gained increased attention in the scientific community in recent years. However, the number of functionally characterized small proteins, especially in archaea, is still limited. Here, by using biochemical and genetic approaches, we demonstrate a crucial role of the small protein sP36 in the nitrogen metabolism of M. mazei, which modulates the ammonium transporter AmtB1 according to nitrogen availability. This modulation might represent an ancient archaeal mechanism of AmtB1 inhibition, in contrast to the well-studied uridylylation-dependent regulation in bacteria.
Show more [+] Less [-]Deutsche Forschungsgemeinschaft (DFG) SCHM1052/20-1+2 Katrin Weidenbach, Ruth A. Schmitz, Tim Habenicht; MEC | Agencia Estatal de Investigación (AEI), PID2019-110900GB-I00, Monica Balsera
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