Arabidopsis thaliana telomeres exhibit euchromatic features
2011
Vaquero-Sedas, M.I. | Gámez-Arjona, Francisco M. | Vega-Palas, Miguel A.
Telomere function is influenced by chromatin structure and organization, which usually involves epigenetic modifications. We describe here the chromatin structure of Arabidopsis thaliana telomeres. Based on the study of six different epigenetic marks we show that Arabidopsis telomeres exhibit euchromatic features. In contrast, subtelomeric regions and telomeric sequences present at interstitial chromosomal loci are heterochromatic. Histone methyltransferases and the chromatin remodeling protein DDM1 control subtelomeric heterochromatin formation. Whereas histone methyltransferases are required for histone H3K9 2Me and non-CpG DNA methylation, DDM1 directs CpG methylation but not H3K92Me or non-CpG methylation. These results argue that both kinds of proteins participate in different pathways to reinforce subtelomeric heterochromatin formation. © The Author(s) 2010. Published by Oxford University Press.
Show more [+] Less [-]Spanish Ministry of Education and Science grants (BFU2004-01560/BMC, BFU2008-02497/BMC and GEN2003-20859-C03-03/INTER); FEDER funds (European Regional Development Funds). Funding for open access charge: Instituto de Bioquícmica Vegetal y Fotosíntesis. The authors thank Judith Bender and Ingo Hoffmann for their kind gift of suvh4-6 and ddm1 seeds. The authors also thank Carmen López for help with the analysis of subtelomeric heterochromatin and Felix Prado and Andrés Aguilera for critical reading of the manuscript.
Show more [+] Less [-]Peer Reviewed
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Instituto de Bioquímica Vegetal y Fotosíntesis