Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments
2009
Portillo Guisado, María del Carmen | González Grau, Juan Miguel | Ministerio de Educación y Ciencia (España)
Simple and rapid methods for the quantification of DNA, RNA and proteins using specific fluorescent dyes are proposed for the comparison and monitoring of microbial communities from the environment. The purpose of this study was the use of straightforward in situ methods which voided the need for preservation of samples and the risk of potential degradation and quantitative changes during transportation. Aside from this, methods used to obtain information on environmental microbial communities are generally time-consuming and present certain difficulty above all when working on solid substrates such as soils and rocks. New generation fluorescent dyes that bind specifically to DNA, RNA and proteins allow simple and rapid estimates of these biomolecules in crude environmental samples.
Show more [+] Less [-]The authors acknowledge support through project CGL2006-11561/BTE from the Spanish Ministry of Education and Science.
Show more [+] Less [-]Peer reviewed
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