Lead tolerance and accumulation in Hirschfeldia incana, a Mediterranean Brassicaceae from metalliferous mine spoils.
2013
Auguy, Florence | Fahr, Mouna | Moulin, Patricia | Brugel, Anaïs | Laplaze, Laurent | Mzibri, Mohamed El | Filali-Maltouf, Abdelkarim | Doumas, Patrick | Smouni, Abdelaziz | Diversité, adaptation, développement des plantes (UMR DIADE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie]) | Centre National de l'Energie, des Sciences et des Techniques Nucléaires (CNESTN) ; Laboratoire de Biotechnologie des Plantes | Laboratoire Mixte International ; Université Mohammed V de Rabat [Agdal] (UM5) | Laboratoire de Physiologie et Biotechnologie Végétale ; Université Mohammed V de Rabat [Agdal] (UM5) | Institut de recherche pour le développement [Dakar, Sénégal] (IRD Hann Maristes) | UMR - Interactions Plantes Microorganismes Environnement (UMR IPME) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [Occitanie]) | LMI Adaptation des Plantes et microorganismes associés aux Stress Environnementaux [Dakar] (LAPSE) ; Institut de Recherche pour le Développement (IRD) | Laboratoire de Microbiologie et Biologie Moléculaire ; Université Mohammed V de Rabat [Agdal] (UM5) | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | International Joint Laboratory "Laboratoire de Biotechnologie Microbienne et Vegetale"; Centre National de l'Energie, des Sciences et Techniques Nucleaires (CNESTEN)/Institut de Recherche pour le Developpement (IRD)/Universite Mohamed V Agdal, Rabat, Morocco; IRD through a BESCD
International audience
Show more [+] Less [-]English. Lead is a heavy metal of particular concern with respect to environmental quality and health. The lack of plant species that accumulate and tolerate Pb is a limiting factor to understand the molecular mechanisms involved in Pb tolerance. In this study we identified Hirschfeldia incana, a Brassicaceae collected from metalliferous mine spoils in Morocco, as a Pb accumulator plant. H. incana exhibited high Pb accumulation in mine soils and in hydroponic cultures. Major Pb accumulation occurred in the roots and a part of Pb translocated from the roots to the shoots, even to the siliques. These findings demonstrated that H. incana is a Pb accumulator species. The expression of several candidate genes after Pb-exposure was measured by quantitative PCR and two of them, HiHMA4 and HiMT2a, coding respectively for a P1B-type ATPase and a metallothionein, were particularly induced by Pb-exposure in both roots and leaves. The functional characterization of HiHMA4 and HiMT2a was achieved using Arabidopsis T-DNA insertional mutants. Pb content and primary root growth analysis confirmed the role of these two genes in Pb tolerance and accumulation. H. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique