Introgression and mapping of novel resistance genes from the secondary genepool of barley, Hordeum bulbosum
2004
Ruge, Brigitte | Linz, A. | Habekuß, Antje | Flath, Kerstin | Wehling, Peter
Enhanceme??t of the genetic diversity in our crops is of a major importance especially in respect to disease resistance. Hordeum bulbosum represents the secondary gene pool of barley (Hordeum vulgare L.) and is a potential source of desirable agronomic-trait genes. Despite the existence of crossing barriers, recombination events between the two Hordeum genomes may occur and lead to introgressions of H. bulbosum genomic segments which carry novel major resistance genes. Telomeric introgressions have been identified that confer resistance to the soil-borne virus complex (BaMMV, Ba YMV-1, -2) as well as to leaf rust and to powdery mildew, respectively. Based on diploid resistant recombinants mapping population were developed. For each of the resistances, a monogenical, dominant inheritance could be demonstrated. Analysis of molecular anchor markers led to the localization of virus-resistance genes on barley chromosomes 6HS and 2HL, as well as leaf-rust and powdery-mildew resistance genes on 2H and 5HL. PCR markers were developed which are suited for routine use in breeding programmes. A diagnostic marker was obtained from a cDNA-AFLP analysis that cosegregated with the soil-borne virus resistance gene Ryml4Hb.
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Publisher Agricultural Res. Inst
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