Enzyme-linked immunosorbent assay for detection of bluetongue virus antibodies
1981
Hübschle, Otto | Lorenz, Rolf J. | Matheka, Heinz-Dietrich
German. The immune response to bluetongue virus in sheep and cattle was studied by applying a newly developed indirect enzyme-linked immunosorbent assay (ELISA). Purified virus obtained by sucrose gradient centrifugation was used at a concentration of 0.01 optical density units (formula: see text) to coat individual wells (200 microliter) of a microtitration plate. Dilution of antigen was performed in 0.05 M carbonate buffer, pH 9.6, and adsorption lasted for at least 16 hours at 4 C. Coated plates retained their activity for 10 weeks when stored at 4 C. Sera recovered from experimentally infected sheep and cattle were tested together with known negative sera. A good correlation between results was obtained with the modified complement-fixation test and the ELISA; however, the ELISA proved to be more sensitive. The group specificity of the ELISA was proven by testing various type-specific sheep and cattle immune sera. The ELISA has potential for the detection of group-specific antibodies to bluetongue virus infection.
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Bibliographic information
Publisher American Veterinary Medical Association (AVMA)
ISSN 0002-9645 | 1943-5681This bibliographic record has been provided by Friedrich-Loeffler-Institut
