Reorganisation of the caecal extracellular matrix upon Salmonella infection—Relation between bacterial invasiveness and expression of virulence genes
2009
Berndt, Angela | Müller, Jens | Borsi, Laura | Kosmehl, Hartwig | Methner, Ulrich | Berndt, Alexander
Interactions of Salmonella (S.) Outer membrane Structures with extracellular matrix (ECM) of host tissues scent to be crucial for bacterial adhesion and invasion. To evaluate the relationship between the ECM and bacterial invasiveness. the reorganisation of fibronectin, tenascin-C and laminin after Salmonella exposure ill vivo, the Salmonella adhesiveness to ECM proteins ill vitro and the Virulence gene expression upon co-cultivation of salmonella and ECM proteins were elucidated for two Salmonella strains with different capabilities to enter the intestinal mucosa. Immunohistochemistry and confocal microscopy showed that the infection of day-old chicks using either the highly invasive S. Enteritidis (SE) or the nearly non-invasive S. Infantis (SINF) strain was associated with an invasion-dependent reorganisation of fibronectin and tenascin-C in the caecal wall. Compared to SINE clustered formations of SE were localised within and attached to the fibronectin and tenascin-C scaffold in the lamina propria indicating a relevance of ECM for bacterial dissemination ill lower regions of the mucosa. In adhesion assays, SE was, indeed, significantly more adhesive to the matrix proteins than SINF. The attachment was accompanied by all increased fliC mRNA expression in SE demonstrated by microarray analysis as well as quantitative real-time RT-PCR. The data suggest a relationship between the capability of Salmonella serovars to interact with matrix proteins and to disseminate in gut mucosa perhaps in consequence of a matrix-mediated upregulation of the Salmonella motility gene fliC. (C) 2008 Elsevier B.V. All rights reserved
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Publisher Elsevier Science
ISSN 1873-2542 | 0378-1135This bibliographic record has been provided by Friedrich-Loeffler-Institut