Variation in thepsbC gene region of gymnosperms and angiosperms as detected by a single restriction site polymorphism
1997
Ziegenhagen, Birgit | Fladung, Matthias
This paper reports on a PCR-RFLP analysis in a chloroplast DNA region consisting of coding and intergenic spacer sequences of trnS and the adjacent psbC gene. This region was PCR-amplified in 62 woody plant species, predominantly tree species, that represent a broad systematic range in both gymnosperms and dicotyledonous angiosperms. The amplification products were digested by the restriction endonuclease HaeIII (GGBCC). Fourteen different restriction patterns occurred, 5 of which characterised representatives of the gymnosperms, and 9 angiosperm representatives. A single restriction site polymorphism revealed most of the species to share restriction patterns. Groups formed which showed relationships to plant systematic units. This phenomenon is discussed with regard to the psbC gene and the GGCC motif for tracing species' relationships on a high taxonomic level of gymnosperms and angiosperms.
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Bibliographic information
Publisher Springer
ISSN 1432-2242 | 0040-5752This bibliographic record has been provided by Thünen-Institut
