International round-robin study on the Ames fluctuation test

Reifferscheid,G.; Maes,H.M.; Allner,B.; Badurova,J.; Belkin,S.; Bluhm,K.; Brauer,F.; Bressling,J.; Domeneghetti,S.; Elad,T.; Flückiger-Isler,S.; Grummt,H.J.; Gürtler,R.; Hecht,A.; Heringa,M.B.; Hollert,H.; Huber,S.; Kramer,M.; Magdeburg,A.; Ratte,H.T.; Sauerborn-Klobucar,R.; Sokolowski,A.; Soldan,P.; Smital,T.; Stalter,D.; Venier,P.; Ziemann,C.; Zipperle,J.; Buchinger,S.

International round-robin study on the Ames fluctuation test

2012

An international round-robin study on the Ames fluctuation test [ISO 11350, 2012], a microplate version of the classic plate-incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by 18 laboratories from seven countries. Such a round-robin study is a precondition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. The laboratories tested four water samples (spiked/nonspiked) and two chemical mixtures with and without supplementation of a S9-mix. Validity criteria (acceptable spontaneous and positive control-induced mutation counts) were fulfilled by 92-100%, depending on the test conditions. A two-step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine-square-root transformation to detect significant differences between the test samples and the negative control (NC). A threshold (TH) value based on a pooled NC was then calculated to exclude false positive test results. Statistically, positive effects observed by the William's test were considered negative, if the mean of all replicates of a sample did not exceed the calculated TH. By making use of this approach, the overall test sensitivity was 100%, and the test specificity ranged from 80 to 100%.

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AGROVOC Keywords

air analyse analysis analysis of variance animals assessment berlin biology carcinogen carcinogenicity carcinogens chemicals classification countries detection ecology effects effluents environment evaluation food food safety genotoxicity germany hygiene identification in vitro italy laboratories law legislation metabolism methods mutagenicity mutagens mutation netherlands oecd pollutants population products quality rats rats research risk risk assessment safety salmonella salmonella typhimurium samples sampling science soil stability state strains surface surface water switzerland system testing toxicology waste water wastewater water water quality water samples

Bibliographic information

Published in

Environmental and molecular mutagenesis : journal of The Environmental Mutagen Society | Environ Mol Mutagen

Volume 53 Issue 3 Pagination 185 - 197

Publisher Wiley-Liss

ISSN 0893-6692 | 1098-2280

Other Subjects

Variability; In-vitro; Validation; Level; Mixture; Limit; Marine; Anova; Wistar; Term; Sensitivity and specificity; International; Determination; Water pollutants; Chemical; Alternative; Product; Aquatic sediment; Vitro; Responses; Aquatic; Reproducibility of results; Mutagenicity tests; Typhimurium; Line; Genetic procedures; Salmonella-typhimurium; Sensitivity; Detect; Effect; Bfr; Risk-assessment; Specificity; Iso; Difference; Variation; Comparison; Mixtures; Surface-water; Compound; Round-robin study; Compounds; Studies; Control; Induction; Assay; Genotoxic; Ames fluctuation test; Chemical; Article; Cell; Guideline; Industrial waste; Ecosystem; Standardization; Collaborative study; Surface waters; Micronucleus test; Standardisation; Waters; Male; Treatment; Study; Method; Reference; Controlled study; Approach; Waste products; Criteria; Chemical accident; Environmental; Hazard; Cell-line; Review; Strain; Protocol; Response; Performance; Cell line

Language

English

Type

Text; Journal Article

In AGRIS since: 2025-02-03

Modification date: 2025-07-17

Format: MODS

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DOI https://www.openagrar.de/receive/bimport_mods_00001343

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International round-robin study on the Ames fluctuation test

2012

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Bibliographic information