High heterogeneity within methicillin-resistant Staphylococcus aureus ST398 isolates defined by Cfr9I macrorestriction-PFGE profiles, spa- and SCCmec-types
2009
Argudin, M.A. | Fetsch, A. | Tenhagen, B.-A. | Hammerl, J. A. | Hertwig, S. | Kowall, J. | Rodicio, M. R. | Käsbohrer, A. | Helmuth, R. | Schroeter, A. | Mendoza, M. C. | Bräunig, J. | Appel, B. | Guerra, B.
German. During recent years, the animal associated methicillin-resistant Staphylococcus aureus clone ST398 has extensively been studied. The DNA of these isolates turned out to be refractory to SmaI-restriction, and consequently, SmaI is unsuitable for subtyping this clone by standard pulsed-field gel electrophoresis (PFGE). Very recently, ST398 DNA was shown to be digested by Cfr9I, a neoschizomer of SmaI. In the present study we employed the Cfr9I-PFGE on 100 German and five Dutch ST398 isolates, and compared their PFGE profiles, protein A gene variable repeat regions (spa-types), and types of the Staphylococcal Cassette Chromosome mec (SCCmec). The isolates from healthy carrier pigs, clinical samples of pigs, dust from farms, milk and meat were assigned to 35 profiles, which were correlated to the SCCmec-type. A dendrogram with the Cfr9I patterns assigned all profiles to two clusters. Cluster A grouped nearly all isolates with SCCmec-type V, and Cluster B comprised all SCCmec-type IVa and V* (a type V variant first identified as III) carriers plus one isolate with SCCmec-type V. Both clusters grouped also MSSA isolates. The association of the majority of isolates with SCCmec-type V in one large cluster indicated the presence of a successful sub-clone within the clonal complex CC398 from pigs, which has diversified. In general, the combination of Cfr9I-PFGE with spa-, and SCCmec-typing demonstrated the heterogeneity of the series analysed, and can be further used for outbreak investigations and traceability studies of the MRSA ST398 emerging clone
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Publisher American Society for Microbiology (ASM) ; PubMed Central
ISSN 0099-2240 | 1098-5336This bibliographic record has been provided by German Federal Institute for Risk Assessment