FORMATION OF SEMI-INTERCALATION COMPLEXES AT EtBr INTERACTION WITH DNA
2003
Antonyan, A. P. | Davtyan, H. G. | Vardevanyan, P. O.
The features of EtBr complex-formation with DNA are investigated. It is shown, that the chromophore ring of ligand can be inserted into a plane between the next bases in one of strands of double-stranded (ds-DNA) (semi-intercalation complex). The binding curves by semi-intercalative interaction of EtBr with DNA are obtained and the parameters of interaction binding constant (K) and the number of the bases (n), corresponding to one binding site, are determined. It is revealed, that EtBr may bind with completely denatured, single-stranded (ss) DNA, thus, the mechanism of interaction of ligand with ss-DNA also has semi- intercalative character, and the binding parameters K and n coincide with those received at semi-intercalative interaction of EtBr with ds-DNA.
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