Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition

The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens.

Instituto de Fisiología y Recursos Genéticos Vegetales

Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina

Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina

Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina

Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina

Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina

Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina

Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina