Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea
2014
Lucena Reyes, Teresa | Ruvira, María Amparo | Macián Rovira, M. Carmen | Pujalte Domarco, María Jesús | Ruiz Arahal, David
Strain 4SM10T, an aerobic marine, Gramnegative, heterotrophic and non pigmented bacterium isolated from seawater from Vinaroz in Castello´n, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence placed the strain within the Roseobacter clade in the family Rhodobacteraceae. Phylogenetic analyses also showed that strain 4SM10T forms a stable clade with species of the genus Roseovarius, being related to Roseovarius nubinhibens ISMT and Roseovarius aestuarii SMK-122T at 97.5 and 97.4 %16S rRNA sequence similarity, respectively. Average Nucleotide Identity (ANI) values, determined as a measure of overall genomic resemblance, confirmed that strain 4SM10T does not belong to the same species as R. aestuarii CECT 7745T and Roseovarius nubinhibens CECT7750T displaying ANI values well below the 95 % boundary for genomic species. Strain 4SM10T requires Na? plus a divalent cation (eitherMg2?or Ca2?) to grow, reduces nitrate to nitrite and uses a large number of amino acids and organic acids (but no carbohydrates) as sole carbon sources. Enzymatic activities displayed in API ZYM tests are alkaline phosphatase, leucine arylamidase and acidphosphatase. Themajor cellular fatty acids were identified as C18:1 x7c and/or C18:1 x6c (67.1 %). The DNA G?C content was determined to be 54.27 mol%. Based on the genotypic and phenotypic data obtained, the name Roseovarius albus sp. nov. is proposed for this novel taxon, with the type strain 4SM10T (=CECT 7450T = KCTC 22653T).
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