No genetic link between E. coli isolates carrying mcr-1 in bovines and humans in France
2025
Haenni, Marisa | Châtre, Pierre | Beyrouthy, Racha | Drapeau, Antoine | François, Pauline | Madec, Jean-Yves | Bonnet, Richard | Unité Antibiorésistance et Virulence Bactériennes (AVB) ; Laboratoire de Lyon [ANSES] ; Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Service Bactériologie [CHU Clermont-Ferrand] ; CHU Gabriel Montpied [Clermont-Ferrand] ; CHU Clermont-Ferrand-CHU Clermont-Ferrand-Pôle Biologie médicale et Anatomie pathologique [CHU Clermont-Ferrand] ; CHU Gabriel Montpied [Clermont-Ferrand] ; CHU Clermont-Ferrand-CHU Clermont-Ferrand | ANR-20-PAMR-0010,Seq2DiAg,Séquençage du génome entier et intelligence artificielle pour caratériser et diagnostiquer la résistance aux antibiotiques et la capacité d'échapper au traitement(2020)
International audience
Show more [+] Less [-]English. Colistin is a last-line antibiotic used to treat severe human infections caused by carbapenemase-producing Gram-negative bacteria. In parallel, colistin has massively been used in the veterinary field so that mcr-1 -positive E. coli have spread worldwide in livestock, potentially constituting a reservoir of colistin-resistant isolates that can be further transmitted to humans.Objectives: In France, the mcr-1 gene was frequently identified in E. coli of bovine origin. This genomic study assessed whether French human mcr-1 -positive E. coli might originate or derive from the bovine reservoir.Material and methods: Human (n = 24) and bovine (n = 127) isolates collected between 2011 and 2019 were included and colistin-resistance was confirmed by MICs. The detection of mcr-1 was performed by PCR. Isolates were short-read whole-genome sequenced and a cgMLST-based phylogeny was constructed. The genetic support of mcr-1 was identified using short-read sequences or Southern blots.Results: The mcr-1 gene was carried by a high diversity of genetic backgrounds, among which ST167 and ST10 were the most widespread. No clonally-related isolates between bovines and humans were observed. In bovines, mcr-1 was identified on IncHI2 and IncX4 plasmids and increasingly on the chromosome, while it was also found on IncI2 and p0111 plasmids in humans. <div>Conclusion:<p>Although similar STs (ST744 and ST88) and plasmid types (IncHI2, IncX4) carried mcr-1 , no hypothesis of a transfer from bovines to humans could be supported by the data. Furthermore, the increasing chromosomal location of mcr-1 over time may reflect an animal-specific evolutionary pathway deserving further investigation.</p></div>
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