Evaluation of the various fungal infection of the skin and gills using pathology, culturing, and molecular fungal DNA sequencing of the freshwater ornamental fish
2024
Mahdi Ebrahimijafari | Mansour bayat | Adel Haghighi Khiabanian Asl | Seyed Jamal Hashemi Hazaveh
In aquaculture, diseases cause tremendous economic losses, and fungal infections are one of the main factors responsible for mortality and economic losses among the ornamental and food fish farming industries. This study focuses on isolating and identifying fungi from different parts of Tehran (I.R. of Iran). The 92 ornamental fish from some pet fish supply stores with clinical signs were selected. The study demonstrated that only 43 of those 92 samples were infected with mold and yeast. The histopathological results revealed that the pathogen established initial infection on and inside the gills, cutaneous and subcutaneous. The special stains were cultured and microscopically examined, followed by PCR and sequencing to detect the fungi. They identified Cladosporium spp. (53%), Candida spp. (18.7%), Penicillium spp. (12.5%), Aureobasidium pullulans (3%), and Rhodotorula yeast (3%). The sequence data were compared with previously published data for identification with the Basic Local Alignment Search Tool (BLAST) via GenBank. According to the presented study, in 14(63%) cases, there was no similar sample in the NCBI.
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