MICROSATELLITE DIVERSITY IN BOS TAURUS, EQUUS CABALLUS AND GALLUS DOMESTICUS BREEDS REARED IN UKRAINE

This study is dedicated to the comparative analysis of the main parameters of microsatellite variability in the populations of animals from different taxa (Bos taurus, Equus caballus, and Gallus domesticus) of different breeds, reared in Ukraine. To investigate microsatellite variability, the following SSR-markers were used: for Bos taurus – TGLA126, TGLA122, INRA023, ETH003, ETH225, BM1824, TGLA227, BM2113, ETH10 and SPS115; for Equus caballus – HTG04, HMS06, AHT04, ASB23, HTG07, HTG06, CA425, VHL20, HMS03, HMS07 and ASB17; for Gallus domesticus – ADL0268, ADL0278, MCW0248, LEI0094 and MCW0216. The results of analyzing the parameter of the average number of alleles per locus (A) were used to determine their least amount in Gallus domesticus (6.56) and the highest one – in Equus caballus (10.76). The observed data are in agreement with the standardization procedure results, based on the rarefaction analysis on the level of 25 animals for each specific species of animals. The highest values of the total genetic diversity (uHe) were notable for Bos taurus (0.835), and the lowest ones – for Gallus domesticus (0.690). These results were confirmed by the Shannon’s index values (1.940 for Bos taurus, 1.886 for Equus caballus and 1.420 for Gallus domesticus) as well as by the number of effective alleles (6.166; 5.614 and 3.848, respectively). The value of genetic subdivision (differentiation) according to Fst values fluctuated depending on the taxon and amounted to 0.119 for Gallus domesticus; 0.043 for Equus caballus and 0.03 for Bos taurus. Genetic differentiation between the populations, evaluated by the analysis of molecular variance (AMOVA), was in the range from 3 to 14 % for different taxa.