Genetic fine-mapping of anthracnose disease-resistance allele Co-14 present in the Andean common bean cultivar AND 277.
2022
LIMA, L. R. L. | GONÇALVES-VIDIGAL, M. C. | BISNETA, M. V. | VALENTINI, G. | VIDIGAL FILHO, P. S. | MARTINS, V. DA S. R. | SOUZA, T. L. P. O. DE | LAÍZE RAPHAELLE LEMOS LIMA; MARIA CELESTE GONÇALVES-VIDIGAL; MARIANA VAZ BISNETA; GISELI VALENTINI; PEDRO SOARES VIDIGAL FILHO; VANUSA DA SILVA RAMOS MARTINS; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF.
Anthracnose (ANT) is among the common bean fungal diseases responsible for significant yield and grain quality losses. Durable genetic resistance is the primary ANT control method due to the pathogen's high variability. Genetic studies showed that the common bean chromosome Pv01 contains multiple disease resistance genes, including the ANT resistance loci Co-1 (Co-12, Co-13, Co-14, Co-15, Co-1HY, and Co-1x), Co-AC, Co-14, Co-Pa, Co-Perla, Co-w, Co-x, and CoPv01CDRK. This work aimed to: (i) perform the genetic fine-mapping of the Co-14 allele present in the cultivar AND 277, using recombinant inbreeding lines derived from the cross between Rudá × AND 277; and (ii) identify candidate resistance genes in the Co-14 allele based on the common bean reference genome. Initially, the Co-14 allele was mapped between the single-nucleotide polymorphism markers ss715645251 and ss715645250 at a distance of 2.0 and 19.6 cM, respectively. Fine-mapping localized Co-14 between the markers ss715645251 and BARCPVSSR01356, spanning a 40.51 kb region at the end of Pv01. Two genes are described within the Co-14 region in the reference genome, Phvul.001G243800 and Phvul.001G243900. The linkage between the Co-14 allele and the markers ss715645251 and BARCPVSSR01356 will be essential for plant breeding programs during the resistance gene transfer to elite cultivars via marker-assisted selection (MAS). Identifying and functionally analyzing candidate resistance genes in this region will allow more efficient MAS by developing accurate markers for ANT resistance.
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