An investigation of the well-water quality: immunosensor for pathogenic Pseudomonas aeruginosa detection based on antibody-modified poly(pyrrole-3 carboxylic acid) screen-printed carbon electrode
2015
Bekir, Karima | Bousimma, Feriel | Barhoumi, Houcine | Fedhila, Kais | Maaref, Abderrazak | Bakhrouf, Amina | Ben Ouada, Hafedh | Namour, Philippe | Jaffrezic-Renault, Nicole | Ben Mansour, Hedi | Laboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir] (LATVPEP) ; Faculté de Pharmacie [Monastir] (FPHM) | Laboratoire des Interfaces et Matériaux Avancés [Monastir] (LIMA) ; Faculté des Sciences de Monastir (FSM) ; جامعة المنستير - Université de Monastir - University of Monastir (UM)-جامعة المنستير - Université de Monastir - University of Monastir (UM) | Res Unit, Anal & Proc Appl Environm ; جامعة المنستير - Université de Monastir - University of Monastir (UM) | Milieux aquatiques, écologie et pollutions (UR MALY) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | River Monitoring & Management - Suivi et gestion des rivières (2014-2016) ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | ISA-Interfaces & biosensors - Interfaces & biocapteurs ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)
[Departement_IRSTEA]Eaux
Show more [+] Less [-]International audience
Show more [+] Less [-]English. In this report, we describe a new immunosensor designed for the detection and the quantification of Pseudomonas aeruginosa bacteria in water. The developed biosensing system was based on the immobilization of purified polyclonal anti P. aeruginosa antibodies on electropolymerized poly(pyrrole-3-carboxylic acid)/glassy carbon electrode. The building of the immunosensor step by step was evaluated by electrochemical measurements such as cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical signature of the immunosensor was established by the change of the charge transfer resistance when the bacteria suspended in solution became attached to the immobilized antibodies. As a result, stable and high sensitive impedimetric immunosensor was obtained with a sensitivity of 0.19 k Omega/decade defined in the linear range from 10(1) to 10(7) CFU/mL of cellular concentrations. A low detection limit was obtained for the P. aeruginosa bacteria and a high selectivity when other bacteria were occasioned as well as Escherichia coli. The developed immunosensor was applied in detecting pathogenic P. aeruginosa in well-water.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique
