" Comparación de tres métodos para el aislamiento de Adn en girasol."
2004
Castillo Reyes, Francisco | García Villanueva, Alma Patricia | Gómez Martínez, Martha | Reyes Valdés, Manuel Humberto
"Para obtener ADN de buena calidad en una especie en particular, frecuentemente se requiere del ensayo de diferentes métodos de aislamiento. Se compararon tres métodos de aislamiento de ADN en girasol en términos de su concentración, pureza y comportamiento electroforetico: Puregene® DNA purification kit (Gentre systems), Doyle y Doyle (1990) y Graham et al. (1994). Dos de estos métodos resultaron aceptables para aislamiento de ADN en términos de razón de absorbancia, concentración y aspecto electroforético. Se seleccionó el método Doyle y Doyle (1990) por tener un menor costo."
Show more [+] Less [-]"Comparison of three methods for DNA isolation in sunflower. To obtain good quality DNA in a given species testing of different isolation methods is often required. Three methods of DNA isolation were tested in sunflower, and evaluated according to the concentration, purity and electrophoretic behavior of Puregene® DNA purification kit (Gentre systems), Doyle and Doyle (1990) and Graham et al. (1994). Two of those methods were acceptable for DNA isolation in terms of absorbance ratio, concentration and electrophoretic aspect. The method of Doyle and Doyle (1990) was selected for having a lower cost."
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Universidad Autónoma Agraria Antonio Narro