Callus induction and establishment of efficient regeneration system for a newly developed line of Ananas comosus (L.) Merrill
2025
Cheng-Li Liu | Fu-Rong Xu | Heng-Yu Huang
In vitro culture method is developed for propagation of a new line of Ananas comosus (L.) Merrill selected from a spontaneous mutant of cultivar Yellow Mauritius. The stem with leaves base obtained from sucker buds was selected as explants. The effects of type and concentration of different plant growth regulators on callus induction, adventitious bud formation and plant regeneration were investigated by the single factor, complete combination and L9 (34) orthogonal experiment. A large number of adventitious buds differentiated on Murashige and Skoog (MS) medium with 4.0 mg·L–1 6-BA, 1.0 mg·L–1 NAA and 1.0 mg·L–1 KT, reaching differentiation coefficient over 12.8. Browning of callus after 2–3 subcultures was eliminated by the application of 1.0 g·L–1 of activated carbon in the optimal medium, which didn’t significantly reduce differentiation coefficient. The main shoots in adventitious buds were higher in number and stronger suitable for rooting in culture. Hundred percent rooting in vitro was achieved on half-strength MS medium with 1.0 mg·L–1 NAA. The survival rate of the tissue culture-raised plants was 100%. The methods developed and defined can be used not only for improved the yield of in vitro plants, but also for shortening the whole culture cycle.
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