EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS
2016
Majid Hameed Mohammed
Human platelet lysates (HPL) have been described as appropriate cell culturesupplement for cultures of mesenchymal stem cells (MSCs). The increase in demandfor harmless and animal-free cultures of MSCs is related to the potential applicationof MSCs grow in human source supplementsIn this study, human platelet lysates are derived from fresh and expired blooddonor platelet concentrates. Mouse bone marrow-derived mesenchymal stem cellswere cultured with one of three culture supplements; fetal bovine serum, lysates fromfresh and expired prepared human platelet concentrates. The impacts of these plateletderived culture supplements on basic mesenchymal stem cell characteristics wereevaluated. All cultures preserved the mesenchymal stem cells surface markerexpression, growth carves and viability test. The result demonstrated thatmesenchymal stem cells complemented with platelet lysates proliferated faster thanconventional FBS cultured cells and increased the expression of the cells. Inconclusion, the use of fresh and expired platelet units from blood banks to prepareplatelet lysates for the culture of MSCs is appropriate as well as keep cells phenotypecharacteristics and also shortens culture time by increasing their growth.
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