MUTANT OF ALOCAL ISOLATE OF LACTOBACILLUS PLANTARUMBY UV LIGHTIN COMPARISION WITH ASTANDARD STRAIN THE EXTENT OF ITSABILITY TO LOWER CHOLESTEROL LEVELS1
2016
Sinan J. Abdul-Abbas | Aswan H. Aboud Al-bayyar | Adnan E. Al-Badran
Local strain bacteria of Lactobacillus plantarum IRQ12 isolated from cucumbersativuspickle registered in (GenBank LN871447) and another standard strain, IRQ12strain was mutated by UV light (254 nm) for (50 cm) distance for different periods 20,25, 30, 35 and 60 second, RAPD – PCR test was done to investigate the effect of UVlight onL.plantarum genes and determine the genetic distant. The ability ofstandard andlocal mutated bacteria to decrease the amount of cholesterol was determined in MRSbroth–EY mediawhich was (4.6 mg/ml), the cholesterol amount was decreased at 25swhich was0.35 mg/ml in a decreased percentage 92.3%, followed by 60s and 35s whichwere 0.43and 0.45mg/ml in a decreased percentage 90.6% and 90.2%, respectively.
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