STUDY THE EFFECT OF FLAX LIGNAN EXTRACT OF LINUM USITATISSIMUM AND CONJUGATED ESTROGEN ON PHYSIOLOGICAL PARAMETERS IN FEMALE RATS

This study was carried out in the animal house of the College of Veterinary Medicine /Basrah University to evaluate the effect of lignan extract of flax seed on haematological, biochemical parameters and histological examination in female rats, compared with conjugated estrogen drug. For this purpose, eighteen female rats weighed (180-200g) and aged (4-5 months) was divided into 3 equal groups (6 rats / group) for 14 days. First group: Drenched 1ml of normal saline (0.9% of NaCl)for 14 days. Second group: Drenched 0.10mg/kg B.W of conjugated estrogen dissolved in normal saline (0.9% of NaCl) for 14 days. Third group: Drenched 20mg/kg B.W of lignan extract of flaxseeds dissolved in normal saline (0.9% of NaCl)for 14 days. Blood samples were collected from heart by cardiac puncture from all experimental animals. These samples are used for the measurement of haematological and biochemical parameter as well as hormonal assy. Thyroid glands, liver, kidneys, ovaries and uterus were removed for histopathological study. The results revealed that, the female rats treated with lignan extract of flax seeds showed an increase in body weight. The result also showed that treated female rats with phytoestrogen had no significant effect on blood parameters except a significant effect on lymphocytes. (P≤ 0.05) in serum lipid profile except HDL compared to control. Moreover the results of biochemical parameters were indicated the affected in female rats treated with lignan extract that the revealed a significant(P≤ 0.05) decrease in serum lipid profile except HDL-Ch of female rats treated with lignan extract compared with the control group. Also the treatment with lignan extract caused significant (P≤ 0.05) increase in LH hormone, estradiol and progesterone compared with .While it has no significant effect on FSH. The treatment showed some ameliorative effect on histological structure of studied organs.