23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY
2018
Mohammed Zyarah Eskander
Polysaccharide was extracted from the leaves of (Molokhia) Jew’s-mallow by hotwater extraction using trichloro acetic acid(TCA) to removal of protein from the polysaccharide ,precipitation with ethanol, and obtained a polysaccharide yield from 4.2% based on wet weight source. The biochemical composition of the polysaccharide contains total carbohydrate,sulfateandprotein77.6%, 8.6%, 0.63%, respectively. Phytochemical tests were carried out for polysaccharide. The previous studies indicated the presence of glycoside, phenol and tannin in polysaccharide from Jew’smallow. However, the presence of both alkaloids and saponins was not observed .The Fourier transform infrared (FTIR) spectral analysis of the polysaccharides consist of carboxyls and sulfate groups.The anticoagulant activity of polysaccharide was evaluated by activated partial thromboplastin time (APTT) and prothrombin time (PT) assays with respect to heparin The results obtained by APTT assay, through the increase in coagulation time, the increase in blood coagulation time with increased polysaccharide concentration, the 100 μg/ml polysaccharide concentration had the longest time of 210Sce and was compatible with the concentration of1000 (IU/ ml) of heparin.However the time of PT did not have any apparent effectiveness in increasing the time of coagulation at all concentration compared with heparin.
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