25- BACTERIOLOGICAL ISOLATION AND EVALUATION OF THE ANTIMICROBIAL RESISTANCE OF ENTEROBACTERIAL ISOLATES FROM GALL BLADDER OF SLAUGHTERED CATTLE
2018
Ashwaq Raheem Nazzal
The increasing prevalence of antimicrobial resistance in meat-producing animals, especially ruminants, represents a major problem for human and animal and also could increase the patient's morbidity and mortality. The gallbladder may be a sit of persistence and a source for fecal shedding of certain enteric food-borne pathogen resistant to many antimicrobial agents. In the current study 80 samples (32 bile, 48 epithelium) were examined to isolate the enteric pathogen;AII samples were cultured on primary and selective. The frequency of isolation of microorganisms was (60% )in the epithelium and (40% ) in the bile. The major pathogen isolated were (68.57%) Proteus spp. (21.25%)E-coli ,(7.5)Citrobacter ,(1.25) Psudomnas. and(1.25)Klebsiella.The antibiotic resistance was determined by Kirby-bauer disc diffusion method using 10 of routine and practical antibiotics. In antimicrobial testing from both bile and gallbladder epithelium showed sensitivity to the following antimicrobial :amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin and ciprofloxacin. In conclusion , the current study provide helpful insights into the prevalence of food source pathogens. High level of antibiotic resistance in proteus spp and Ecoli that could transmit to humans through meat and meat products need for monitoring system on the incidence and antimicrobial susceptibility of enteric pathogens in meat animals in slaughterhouses.
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