HISTOPATHOLOGICAL STUDY OF PLATELETE RICH PLASMA FOR TREATMENT OF INDUCED CUTANEOUS ULCER IN DIABETIC RABBITS INDUCED BY STREPTOZOTOCIN
2019
The present study investigates the effect of platelets rich plasma (PRP) on the healing of skin ulcers in rabbits in which diabetes mellitus (DM) was induced using Streptozotocin. The study was applied on 12 adult rabbits of both sexes with a mean weight of 1000-1500 g. They were divided randomly into two equal groups (six rabbits per group). DM was induced in two groups by injected Streptozotosin 65 mg / kg B.W IV. Ulcers were made in 3 cm length and 2 cm width in all rabbits in the gluteal region under the general Anesthesia by mixture of xylazine hydrochloride and ketamine hydrochloride in sterile conditions. The first group was used as control; the second group was treated with platelets rich plasma on the site of ulcers. The first group in the 7 days, showed large ulcers and pus; in the 14 days, were ulcers continued with thickened epidermal. The second group, in the 7days, showed skin ulcers with dermal thickening and a beginning of skin formation. In the 14th days, the treated group showed small ulcer remaining with dermal fibrosis.ELISA results have been shown the effect of platelets rich plasma on tumor necrosis factor-alpha (TNFα) concentration in the blood after 10 days of skin lesions inducing. The natural concentration of TNFα is 6.4 pg/ml in range (4.2-7.9) pg/ml. The first group had a concentration below 5.3 pg/ml, due to the DM. The second group was highly concentrated in 10.4 pg/ml. The results of the histopathological and ELISA confirmed that there are marked healing and elevating TNFα concentration in the treated group.
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