MOLECULAR CHARACTERISATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE USING P32 AND RPO30 GENES IN DUHOK PROVINCE, KURDISAN REGION OF IRAQ.
2020
Ramadhan Ado Khanamir , Jivan Qasim Ahmed, Meqdad Saleh Ahmed, Karwan Idrees Jarjees .
Lumpy skin disease (LSD) is a contagious viral infection of cattle caused by lumpyskin disease virus (LSDV). This infection has a huge economic impact on the cattle industryas a result of skin damage, inflammation of mammary glands and decrease milk production,reduces reproduction and even death as a consequence of secondary bacterial infection. Iraqis considered as an endemic country by OIE that continues breaking throughout differentparts of the country. The aims of this research work was to detect and molecularcharacterization of LSDV in Duhok province for the first time through using a phylogeneticapproach of P32 and RPO30 genes. The results showed that LSDV was detected andidentified over using PCR and sequencing and the isolate 1 LSDV/Duhok/Kurdistan/2019,isolate 2 LSDV/Duhok/Kurdistan/2019 and isolate 3 LSDV/Duhok/Kurdistan/2019 wereclustered in LSDV lineage for both P32 and RPO30 genes. The phylogenetic analysis ofthese isolates showed a close relationship with the previously published isolates based on P32gene sequences, while this study is the first study to publish genetic information on RPO30gene of the Iraqi isolates and compare them to the other publicly available data sets of othercountries. To sum up, the findings of this paper could be beneficial to illustrate the spreadingnature of LSDV in Iraq and mainly in the Kurdistan region of Iraq. Furthermore, offer thetheoretical references for control and prevention of LSDV infections in future.
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