A new method for determining enzyme kinetic constants | A new method for determining enzyme kinetic constants
2025
Martín Sarmiento, Roberto | Burgos González, Justino
English. A new procedure is described for calculating kinetic parameters of reactions which obey Michaelis-Menten and derived equations. The performance of this method at several substrate concentration ranges was compared with that of the plots of l/v versus l/s, s/v, versus s and v versus v/s and with the procedures of WILKINSON and EISENTHAL & CORNISH-BOWDEN by using simulated rate data containing errors of constant absolute magnitude, constant relative magnitude and intermediate, with and without outliers in each case. The method proved to be excelent in accuracy and precision in all the explored situations.
Show more [+] Less [-]Spanish; Castilian. Se describe un nuevo procedimiento para calcular los parámetros cinéticos de las reacciones que obedecen a ecuaciones del tipo de la de Michaelis-Menten y se compara su rendimiento, a varios rangos de concentraciones de sustrato, con el de las representaciones de l/v en función de l/s. s/v frente a s y v en función de v/s y. con el de los métodos de WILKINSON y de EISENTHAL y CORNISH-BOWDEN; a este último objeto se utilizaron datos experimentales simulados en un ordenador, conteniendo errores de magnitud absoluta constante, de magnitud relativa constante e intermedios. El método proporcionó excelentes resultados en todas las circunstancias investigadas.
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